Composite

Part:BBa_I723023:Design

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)


Luciferase reporter activated by xylene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal NheI site found at 2485
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 530
    Illegal BglII site found at 1992
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1087
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1183
    Illegal BsaI.rc site found at 3183


Design Notes

Be aware that there is a PstI site in the XylR gene. Requires site directed mutagenesis

Source

[Christine/Maia]

References